[Biomarkers related to cognitive dysfunction in APP/PS1 mice based on non-targeted metabonomics and intervention mechanism of Huanglian Jiedu Decoction].

小桶 代谢组学 代谢途径 药理学 生物化学 脂肪酸代谢 轨道轨道 犬尿氨酸途径 化学 新陈代谢 生物 犬尿氨酸 氨基酸 生物信息学 色氨酸 质谱法 基因 色谱法 转录组 基因表达
作者
Linna Wang,Xinru Gu,Nan Si,Hongjie Wang,Yanyan Zhou,Baolin Bian,Haiyu Zhao
出处
期刊:PubMed 卷期号:47 (22): 6117-6126 被引量:2
标识
DOI:10.19540/j.cnki.cjcmm.20220705.401
摘要

Through the non-targeted metabonomics study on endogenous substances in APP/PS1 transgenic mice, this paper aimed to discover biomarkers related to APP/PS1 mice with cognitive dysfunction, and find targets of Huanglian Jiedu Decoction(HLJDD) in the treatment of Alzheimer's disease(AD) and its mechanism. The brain tissue and serum metabolic mass spectrometry of mice were analyzed by ultra-high performance liquid chromatography-Orbitrap mass spectrometry(UPLC-Orbitrap MS). Through partial least squares-discriminant analysis(PLS-DA) and orthogonal partial least squares-discriminant analysis(OPLS-DA), the metabolic data of the normal group, the model group, the high-dose and low-dose HLJDD groups, and the berberine group were compared and analyzed to screen out potential biomarkers, and the relevant metabolic pathways were constructed with the help of the Kyoto Encyclopedia of Genes and Genomes(KEGG) database. Forty-five potential endogenous metabolites were identified, including 13 in brain and 35 in serum, among which leukotriene B4, tyrosine, and adenosine were expected to be differential metabolites related to cognitive function. HLJDD recalled 22 differential metabolites, and the pathways mainly involved in aminoacyl-tRNA biosynthesis, valine, leucine and isoleucine biosynthesis, pantothenic acid and coenzyme A biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, and arachidonic acid metabolism. These pathways suggested that the main mechanism of HLJDD in the intervention of AD was to inhibit central and peripheral inflammation, and regulate energy metabolism, fatty acid metabolism, and amino acid metabolism. HLJDD has a certain effect on the improvement of cognitive dysfunction, and regulates relative pathways by recalling endogenous differential metabolites, which helps to further discover the biomarkers of AD and clarify the intervention mechanism of HLJDD in the treatment of AD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
欣慰的星月完成签到,获得积分10
2秒前
FashionBoy应助潇湘雪月采纳,获得10
5秒前
传奇3应助leanne采纳,获得10
5秒前
大模型应助Heartlark采纳,获得10
7秒前
9秒前
moxi摩西完成签到,获得积分10
9秒前
卷卷完成签到,获得积分10
11秒前
时笙发布了新的文献求助10
12秒前
12秒前
pterion完成签到,获得积分10
12秒前
12秒前
14秒前
哒哒完成签到,获得积分10
16秒前
16秒前
循环发布了新的文献求助10
16秒前
幽默毛衣发布了新的文献求助10
19秒前
21秒前
循环完成签到,获得积分10
21秒前
leanne发布了新的文献求助10
21秒前
22秒前
量子星尘发布了新的文献求助10
22秒前
23秒前
23秒前
开灯人和关灯人完成签到,获得积分20
24秒前
Stardust发布了新的文献求助10
25秒前
26秒前
FashionBoy应助爱笑晓曼采纳,获得10
27秒前
张雯思发布了新的文献求助10
28秒前
Priority完成签到,获得积分10
29秒前
光热效应发布了新的文献求助30
29秒前
风之星给风之星的求助进行了留言
29秒前
29秒前
ASH发布了新的文献求助10
29秒前
OxO完成签到,获得积分10
29秒前
30秒前
搜集达人应助快乐一江采纳,获得10
30秒前
31秒前
leanne完成签到,获得积分20
32秒前
幽默毛衣完成签到,获得积分10
33秒前
晨曦完成签到,获得积分10
33秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989390
求助须知:如何正确求助?哪些是违规求助? 3531487
关于积分的说明 11254109
捐赠科研通 3270153
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809174