亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

[Biomarkers related to cognitive dysfunction in APP/PS1 mice based on non-targeted metabonomics and intervention mechanism of Huanglian Jiedu Decoction].

小桶 代谢组学 代谢途径 药理学 生物化学 脂肪酸代谢 轨道轨道 犬尿氨酸途径 化学 新陈代谢 生物 犬尿氨酸 氨基酸 生物信息学 色氨酸 质谱法 基因 色谱法 转录组 基因表达
作者
Linna Wang,Xinru Gu,Nan Si,Hongjie Wang,Yanyan Zhou,Baolin Bian,Haiyu Zhao
出处
期刊:PubMed 卷期号:47 (22): 6117-6126 被引量:2
标识
DOI:10.19540/j.cnki.cjcmm.20220705.401
摘要

Through the non-targeted metabonomics study on endogenous substances in APP/PS1 transgenic mice, this paper aimed to discover biomarkers related to APP/PS1 mice with cognitive dysfunction, and find targets of Huanglian Jiedu Decoction(HLJDD) in the treatment of Alzheimer's disease(AD) and its mechanism. The brain tissue and serum metabolic mass spectrometry of mice were analyzed by ultra-high performance liquid chromatography-Orbitrap mass spectrometry(UPLC-Orbitrap MS). Through partial least squares-discriminant analysis(PLS-DA) and orthogonal partial least squares-discriminant analysis(OPLS-DA), the metabolic data of the normal group, the model group, the high-dose and low-dose HLJDD groups, and the berberine group were compared and analyzed to screen out potential biomarkers, and the relevant metabolic pathways were constructed with the help of the Kyoto Encyclopedia of Genes and Genomes(KEGG) database. Forty-five potential endogenous metabolites were identified, including 13 in brain and 35 in serum, among which leukotriene B4, tyrosine, and adenosine were expected to be differential metabolites related to cognitive function. HLJDD recalled 22 differential metabolites, and the pathways mainly involved in aminoacyl-tRNA biosynthesis, valine, leucine and isoleucine biosynthesis, pantothenic acid and coenzyme A biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, and arachidonic acid metabolism. These pathways suggested that the main mechanism of HLJDD in the intervention of AD was to inhibit central and peripheral inflammation, and regulate energy metabolism, fatty acid metabolism, and amino acid metabolism. HLJDD has a certain effect on the improvement of cognitive dysfunction, and regulates relative pathways by recalling endogenous differential metabolites, which helps to further discover the biomarkers of AD and clarify the intervention mechanism of HLJDD in the treatment of AD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
9秒前
tlh完成签到 ,获得积分10
16秒前
直觉应助阿金啊采纳,获得30
41秒前
49秒前
55秒前
58秒前
hongtao发布了新的文献求助10
1分钟前
1分钟前
闫雪发布了新的文献求助10
1分钟前
传奇3应助科研通管家采纳,获得10
1分钟前
我是老大应助闫雪采纳,获得10
1分钟前
1分钟前
hongtao完成签到 ,获得积分10
1分钟前
2分钟前
Jasper应助科研通管家采纳,获得10
3分钟前
3分钟前
3分钟前
璐璐侠完成签到,获得积分10
3分钟前
4分钟前
AJ完成签到 ,获得积分10
4分钟前
xiaozou55完成签到 ,获得积分10
4分钟前
binyao2024完成签到,获得积分10
6分钟前
光亮静槐完成签到 ,获得积分10
6分钟前
lrl350495627发布了新的文献求助10
7分钟前
lrl350495627完成签到,获得积分10
7分钟前
GingerF应助科研通管家采纳,获得50
7分钟前
FashionBoy应助andrele采纳,获得10
7分钟前
8分钟前
自然芷文发布了新的文献求助10
8分钟前
8分钟前
我是老大应助自然芷文采纳,获得10
9分钟前
iNk应助吗喽采纳,获得20
9分钟前
研友_VZG7GZ应助科研通管家采纳,获得10
9分钟前
打打应助科研通管家采纳,获得10
9分钟前
Charlie完成签到 ,获得积分10
9分钟前
CodeCraft应助Benhnhk21采纳,获得30
9分钟前
10分钟前
Benhnhk21发布了新的文献求助30
10分钟前
科研通AI2S应助科研通管家采纳,获得10
11分钟前
村口的帅老头完成签到 ,获得积分0
11分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990181
求助须知:如何正确求助?哪些是违规求助? 3532136
关于积分的说明 11256472
捐赠科研通 3271042
什么是DOI,文献DOI怎么找? 1805190
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234