Triple Helix Molecular Switch Cascade Multiple Signal Amplification Strategies for Ultrasensitive Chloramphenicol Detection

A novel self-powered biosensor has been developed for the detection of chloramphenicol (CAP) based on difunctional triple helix molecular switch (THMS)-mediated DNA walkers. The biosensor utilizes the CAP aptamer as the recognition element, a DNA walker and capacitor as dual signal amplification strategies, and a digital multimeter (DMM) as the data readout equipment. In the presence of the target, the CAP aptamer in THMS specifically binds with CAP to release a signal transduction probe (STP) and opens the H1 hairpin structure in the biocathode to trigger the DNA walker and form a double-stranded DNA structure. Then, [Ru(NH