纳米孔
分析物
荧光
纳米技术
生物传感器
聚苯乙烯
材料科学
纳米传感器
封锁
生物物理学
化学
色谱法
聚合物
生物化学
生物
光学
受体
物理
复合材料
作者
Thanh Hoang Phuong Doan,Jasper P. Fried,Wenxian Tang,Daniel Hagness,Ying Yang,Yanfang Wu,Richard D. Tilley,J. Justin Gooding
出处
期刊:Nano Letters
[American Chemical Society]
日期:2024-05-17
卷期号:24 (21): 6218-6224
被引量:1
标识
DOI:10.1021/acs.nanolett.4c00530
摘要
Nanopore sensing is a popular biosensing strategy that is being explored for the quantitative analysis of biomarkers. With low concentrations of analytes, nanopore sensors face challenges related to slow response times and selectivity. Here, we demonstrate an approach to rapidly detect species at ultralow concentrations using an optical nanopore blockade sensor for quantitative detection of the protein vascular endothelial growth factor (VEGF). This sensor relies on monitoring fluorescent polystyrene nanoparticles blocking nanopores in a nanopore array of 676 nanopores. The fluorescent signal is read out using a wide-field fluorescence microscope. Nonspecific blockade events are then distinguished from specific blockade events based on the ability to pull the particles out of the pore using an applied electric field. This allows the detection of VEGF at sub-picomolar concentration in less than 15 min.
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