The use of data independent acquisition based proteomic analysis and machine learning to reveal potential biomarkers for autism spectrum disorder

自闭症谱系障碍 自闭症 蛋白质组学 计算机科学 机器学习 计算生物学 数据科学 人工智能 心理学 生物 发展心理学 遗传学 基因
作者
Huajie Zhang,Xiaoxiao Tang,Chengyun Feng,Yan Gao,Qi Hong,Jun Zhang,Xinglai Zhang,Qihong Zheng,Lin Jing,Xukun Liu,Liming Shen
出处
期刊:Journal of Proteomics [Elsevier BV]
卷期号:278: 104872-104872 被引量:12
标识
DOI:10.1016/j.jprot.2023.104872
摘要

Autism spectrum disorder (ASD) is a complex neurological developmental disorder in children, and is associated with social isolation and restricted interests. The etiology of this disorder is still unknown. There is neither any confirmed laboratory test nor any effective therapeutic strategy to diagnose or cure it. We performed data independent acquisition (DIA) and multiple reaction monitoring (MRM) analysis of plasma from children with ASD and controls. The result showed that 45 differentially expressed proteins (DEPs) were identified between autistic subjects and controls. Among these, only one DEP was down-regulated in ASD; other DEPs were up-regulated in ASD children's plasma. These proteins are found associated with complement and coagulation cascades, vitamin digestion and absorption, cholesterol metabolism, platelet degranulation, selenium micronutrient network, extracellular matrix organization and inflammatory pathway, which have been reported to be related to ASD. After MRM verification, five key proteins in complement pathway (PLG, SERPINC1, and A2M) and inflammatory pathway (CD5L, ATRN, SERPINC1, and A2M) were confirmed to be significantly up-regulated in ASD group. Through the screening of machine learning model and MRM verification, we found that two proteins (biotinidase and carbonic anhydrase 1) can be used as early diagnostic markers of ASD (AUC = 0.8, p = 0.0001). ASD is the fastest growing neurodevelopmental disorder in the world and has become a major public health problem worldwide. Its prevalence has been steadily increasing, with a global prevalence rate of 1%. Early diagnosis and intervention can achieve better prognosis. In this study, data independent acquisition (DIA) and multiple reaction monitoring (MRM) analysis was applied to analyze the plasma proteome of ASD patients (31 (±5) months old), and 378 proteins were quantified. 45 differentially expressed proteins (DEPs) were identified between the ASD group and the control group. They mainly were associated with platelet degranulation, ECM proteoglycar, complement and coagulation cascades, selenium micronutrient network, regulation of insulin-like growth factor (IGF) transport and uptake by insulin-like growth factor binding proteins (IGFBPs), cholesterol metabolism, vitamin metabolism, and inflammatory pathway. Through the integrated machine learning methods and the MRM verification of independent samples, it is considered that biotinidase and carbon anhydrase 1 have the potential to become biomarkers for the early diagnosis of ASD. These results complement proteomics database of the ASD patients, broaden our understanding of ASD, and provide a panel of biomarkers for the early diagnosis of ASD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Ava应助科研通管家采纳,获得10
刚刚
爆米花应助科研通管家采纳,获得10
1秒前
SciGPT应助科研通管家采纳,获得10
1秒前
1秒前
田様应助科研通管家采纳,获得10
1秒前
1秒前
1秒前
1秒前
1秒前
1秒前
1秒前
1秒前
英俊的铭应助科研通管家采纳,获得10
1秒前
灰色与青完成签到,获得积分10
2秒前
bkagyin应助ylw采纳,获得10
2秒前
星辰大海应助kcmat采纳,获得10
3秒前
4秒前
qq完成签到,获得积分10
4秒前
4秒前
4秒前
5秒前
上官若男应助无心的土豆采纳,获得10
6秒前
honeylaker发布了新的文献求助10
6秒前
7秒前
callmecjh发布了新的文献求助10
7秒前
9月有书读发布了新的文献求助10
8秒前
9秒前
英俊的铭应助忧虑的尔容采纳,获得10
10秒前
糖糖完成签到,获得积分10
10秒前
Enoch发布了新的文献求助10
11秒前
科目三应助等待八宝粥采纳,获得10
11秒前
11秒前
亨利公爵完成签到,获得积分10
12秒前
13秒前
krenc完成签到,获得积分10
13秒前
清脆谷槐发布了新的文献求助10
14秒前
J_C_Van发布了新的文献求助10
14秒前
14秒前
江南最后的深情完成签到,获得积分10
15秒前
hr完成签到 ,获得积分10
15秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds第二卷 1200
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038657
求助须知:如何正确求助?哪些是违规求助? 3576306
关于积分的说明 11375198
捐赠科研通 3306108
什么是DOI,文献DOI怎么找? 1819379
邀请新用户注册赠送积分活动 892698
科研通“疑难数据库(出版商)”最低求助积分说明 815066