亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

The use of data independent acquisition based proteomic analysis and machine learning to reveal potential biomarkers for autism spectrum disorder

自闭症谱系障碍 自闭症 蛋白质组学 计算机科学 机器学习 计算生物学 数据科学 人工智能 心理学 生物 发展心理学 遗传学 基因
作者
Huajie Zhang,Xiaoxiao Tang,Chengyun Feng,Yan Gao,Qi Hong,Jun Zhang,Xinglai Zhang,Qihong Zheng,Lin Jing,Xukun Liu,Liming Shen
出处
期刊:Journal of Proteomics [Elsevier BV]
卷期号:278: 104872-104872 被引量:12
标识
DOI:10.1016/j.jprot.2023.104872
摘要

Autism spectrum disorder (ASD) is a complex neurological developmental disorder in children, and is associated with social isolation and restricted interests. The etiology of this disorder is still unknown. There is neither any confirmed laboratory test nor any effective therapeutic strategy to diagnose or cure it. We performed data independent acquisition (DIA) and multiple reaction monitoring (MRM) analysis of plasma from children with ASD and controls. The result showed that 45 differentially expressed proteins (DEPs) were identified between autistic subjects and controls. Among these, only one DEP was down-regulated in ASD; other DEPs were up-regulated in ASD children's plasma. These proteins are found associated with complement and coagulation cascades, vitamin digestion and absorption, cholesterol metabolism, platelet degranulation, selenium micronutrient network, extracellular matrix organization and inflammatory pathway, which have been reported to be related to ASD. After MRM verification, five key proteins in complement pathway (PLG, SERPINC1, and A2M) and inflammatory pathway (CD5L, ATRN, SERPINC1, and A2M) were confirmed to be significantly up-regulated in ASD group. Through the screening of machine learning model and MRM verification, we found that two proteins (biotinidase and carbonic anhydrase 1) can be used as early diagnostic markers of ASD (AUC = 0.8, p = 0.0001). ASD is the fastest growing neurodevelopmental disorder in the world and has become a major public health problem worldwide. Its prevalence has been steadily increasing, with a global prevalence rate of 1%. Early diagnosis and intervention can achieve better prognosis. In this study, data independent acquisition (DIA) and multiple reaction monitoring (MRM) analysis was applied to analyze the plasma proteome of ASD patients (31 (±5) months old), and 378 proteins were quantified. 45 differentially expressed proteins (DEPs) were identified between the ASD group and the control group. They mainly were associated with platelet degranulation, ECM proteoglycar, complement and coagulation cascades, selenium micronutrient network, regulation of insulin-like growth factor (IGF) transport and uptake by insulin-like growth factor binding proteins (IGFBPs), cholesterol metabolism, vitamin metabolism, and inflammatory pathway. Through the integrated machine learning methods and the MRM verification of independent samples, it is considered that biotinidase and carbon anhydrase 1 have the potential to become biomarkers for the early diagnosis of ASD. These results complement proteomics database of the ASD patients, broaden our understanding of ASD, and provide a panel of biomarkers for the early diagnosis of ASD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
量子星尘发布了新的文献求助10
7秒前
24秒前
老石完成签到 ,获得积分10
45秒前
chichqq发布了新的文献求助30
46秒前
54秒前
evil发布了新的文献求助10
1分钟前
1分钟前
珂珂完成签到 ,获得积分10
1分钟前
小蘑菇应助evil采纳,获得10
1分钟前
2分钟前
量子星尘发布了新的文献求助10
2分钟前
2分钟前
2分钟前
Dandelion完成签到,获得积分10
2分钟前
3分钟前
完美世界应助nsc采纳,获得10
3分钟前
量子星尘发布了新的文献求助10
3分钟前
所所应助nsc采纳,获得10
3分钟前
小蘑菇应助nsc采纳,获得50
3分钟前
Ava应助nsc采纳,获得10
3分钟前
隐形曼青应助nsc采纳,获得10
3分钟前
慕青应助nsc采纳,获得10
3分钟前
脑洞疼应助nsc采纳,获得10
3分钟前
善学以致用应助nsc采纳,获得10
3分钟前
ding应助nsc采纳,获得30
3分钟前
Hello应助nsc采纳,获得10
3分钟前
烟花应助nsc采纳,获得10
3分钟前
3分钟前
3分钟前
漫步随心完成签到,获得积分20
4分钟前
NexusExplorer应助科研通管家采纳,获得10
4分钟前
4分钟前
今后应助nsc采纳,获得10
4分钟前
bkagyin应助nsc采纳,获得10
4分钟前
小二郎应助nsc采纳,获得10
4分钟前
Jasper应助nsc采纳,获得10
4分钟前
李爱国应助nsc采纳,获得10
4分钟前
脑洞疼应助nsc采纳,获得10
4分钟前
慕青应助nsc采纳,获得10
4分钟前
天天快乐应助nsc采纳,获得10
4分钟前
高分求助中
The Mother of All Tableaux Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 2400
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
Optimal Transport: A Comprehensive Introduction to Modeling, Analysis, Simulation, Applications 800
Official Methods of Analysis of AOAC INTERNATIONAL 600
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
T/CIET 1202-2025 可吸收再生氧化纤维素止血材料 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3957061
求助须知:如何正确求助?哪些是违规求助? 3503084
关于积分的说明 11111255
捐赠科研通 3234121
什么是DOI,文献DOI怎么找? 1787751
邀请新用户注册赠送积分活动 870762
科研通“疑难数据库(出版商)”最低求助积分说明 802264