Charge-conversion and ultrasound-responsive O-carboxymethyl Chitosan Nanodroplets for Controlled Drug Delivery

Aim: O-carboxymethyl chitosan/perfluorohexane nanodroplets (O-CS NDs) and doxorubicin-loading O-carboxymethyl chitosan nanodroplets were synthesized and functionally tested as drug delivery system in vitro. Materials & methods: The characteristics, charge conversion, stability, cytotoxicity, ultrasound imaging ability, interaction with tumor cells of the nanodroplets and eradication on tumor cells of the doxorubicin-loaded nanodroplets were investigated. Results: O-CS NDs (below 200 nm) achieved higher tumor cellular associations at acidic pH, with great serum stability, pH-dependent charge conversion and good ultrasound imaging ability. Doxorubicin-loading O-carboxymethyl chitosan nanodroplets exhibited strong cytotoxicity on PC-3 cells with ultrasound exposure. Conclusion: These stable, safe and smart O-CS NDs may be a promising approach to improve cell interaction efficiency as an ultrasound imaging and cancer-targeting drug delivery system.