PIN and PILS family genes analyses in Chrysanthemum seticuspe reveal their potential functions in flower bud development and drought stress

Auxin affects almost all plant growth and developmental processes. The PIN-FORMED (PIN) and PIN-LIKES (PILS) family genes determine the direction and distribution gradient of auxin flow by polar localization on the cell membrane. However, there are no systematic studies on PIN and PILS family genes in chrysanthemum. Here, 18 PIN and 13 PILS genes were identified in Chrysanthemum seticuspe. The evolutionary relationships, physicochemical properties, conserved motifs, cis-acting elements, chromosome localization, collinearity, and expression characteristics of these genes were analyzed. CsPIN10a, CsPIN10b, and CsPIN10c are unique PIN genes in C. seticuspe. Expression pattern analysis showed that these genes had different tissue specificities, and the expression levels of CsPIN8, CsPINS1, CsPILS6, and CsPILS10 were linearly related to the developmental period of the flower buds. In situ hybridization assay showed that CsPIN1a, CsPIN1b, and CsPILS8 were expressed in floret primordia and petal tips, and CsPIN1a was specifically expressed in the middle of the bract primordia, which might regulate lateral expansion of the bracts. CsPIN and CsPILS family genes are also involved in drought stress responses. This study provides theoretical support for the cultivation of new varieties with attractive flower forms and high drought tolerance.