内吞作用
吸附
纤维连接蛋白
解吸
化学
生物物理学
细胞生物学
纳米技术
材料科学
生物化学
生物
受体
细胞外基质
物理化学
作者
Junyao Li,Lin Ma,Shilong Mao,Jinfeng Wang,Yanfeng Luo
出处
期刊:Langmuir
[American Chemical Society]
日期:2025-01-19
标识
DOI:10.1021/acs.langmuir.4c02526
摘要
Protein adsorption on biomaterials occurs before cell adhesion. To adapt the properties of biomaterials, adhered cells may utilize and modify adsorbed proteins for survival and function. In this process, the protein–material interfacial force (Fad) is supposed to play vital roles, which, however, has received little attention. Here, we found that rat mesenchymal stem cells (rMSCs) can utilize the adsorbed fibronectin (FN) via reorganization, desorption, or endocytosis, and these utilization processes are regulated by Fad through regulating cell adhesion and force balance between the cell traction force and Fad. Furthermore, protein utilization has an Fad-dependent temporal sequence. On low Fad surface, FN endocytosis might happen prior to FN desorption and aggregation. This work confirms the importance of Fad in protein utilization and provides new insight into the mechanism by which cells process their surrounding ECM proteins, which may help to guide the design of better biomaterials.
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