马铃薯Y病毒科
烟草
生物
内质网
病毒学
大肠杆菌
植物病毒
马铃薯Y病毒
生物化学
病毒
基因
作者
Mengzhu Chai,Lei Li,Yong Li,Yingshuai Yang,Yuting Wang,Xue Jiang,Yameng Luan,Fangfang Li,Hongguang Cui,Aiming Wang,Wensheng Xiang,Xiaoyun Wu,Xiaofei Cheng
标识
DOI:10.1073/pnas.2401748121
摘要
Potyviridae, the largest family of plant RNA viruses, includes many important pathogens that significantly reduce the yields of many crops worldwide. In this study, we report that the 6-kilodalton peptide 1 (6K1), one of the least characterized potyviral proteins, is an endoplasmic reticulum–localized protein. AI–assisted structure modeling and biochemical assays suggest that 6K1 forms pentamers with a central hydrophobic tunnel, can increase the cell membrane permeability of Escherichia coli and Nicotiana benthamiana , and can conduct potassium in Saccharomyces cerevisiae . An infectivity assay showed that viral proliferation is inhibited by mutations that affect 6K1 multimerization. Moreover, the 6K1 or its homologous 7K proteins from other viruses of the Potyviridae family also have the ability to increase cell membrane permeability and transmembrane potassium conductance. Taken together, these data reveal that 6K1 and its homologous 7K proteins function as viroporins in viral infected cells.
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