MHC I级
溶解
细胞毒性T细胞
主要组织相容性复合体
细胞毒性
功能(生物学)
抗原
化学
免疫学
生物
分子生物学
CD8型
细胞生物学
体外
生物化学
作者
Nada Chaoul,Marcello Albanesi
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 41-54
被引量:2
标识
DOI:10.1007/978-1-0716-1507-2_3
摘要
Cytotoxicity is the primary function of CD8+ T-cells, also called cytotoxic CD8+ T lymphocytes (or CTLs). Quantification of this capacity is of major importance in diagnostic and research tools. While phenotypic characterization of CTLs is frequent and easily performed, their function is indeed more difficult to assess. CTLs are responsible for the lysis of cells expressing foreign or modified antigen peptides on their MHC class I molecules. Here we describe the detailed protocol used for the in vitro specific lysis of target cells.
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