维罗细胞
体外
体内
继代培养(生物学)
细胞
细胞培养
生物
病毒学
分子生物学
作者
Ailing Li,Hongyan Wang,Yuelan Zhang,Yang Zhao,Ke Ma,Ying Zhang,Jin Zhang,Hongyang Liang,Shuo Zhao,Hui Wang
出处
期刊:Chinese journal of microbiology and immunology
日期:2011-05-31
卷期号:31 (05): 456-461
标识
DOI:10.3760/cma.j.issn.0254-5101.2011.05.017
摘要
Objective To produce an experimental information for the safety assessment of Vero cells during subculture. Methods Passage and freeze on Vero cells, and Vero cells in different passages in vitro and in vivo tumorigenicity were tested. The protein expression of different Vero cell passages was analyzed. Results Vero cells passaged to p270 and 14 cell banks were developed and stored for future evaluation. In vitro and in vivo tumorigenicity Lest results of Vero cells in different passages were negative. Conclusion Although the tumorigenicity test results in vitro and in vivo process were negative, the protein expression of more than p200 Vero cells were changed, which produced the experimental reference for the safety evaluation of the process during the Vero cell passage.
Key words:
Vaccine cell substrate; Vero cell; Safety of passages; Test of tumorigenicity; Proteomics analysis
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