Immunogenic peptide mimotopes from an epitope of Escherichia coli O157 LPS

模拟电影 大肠杆菌 表位 微生物学 化学 肠杆菌科 生物 抗体 病毒学 生物化学 免疫学 基因
作者
Armando Navarro,Ulises Hernández‐Chiñas,Delia Licona-Moreno,Edgar Zenteno,Alejandro Cravioto,Carlos Alberto Eslava-Campos
出处
期刊:Biochemical Journal [Portland Press]
卷期号:473 (21): 3791-3804 被引量:4
标识
DOI:10.1042/bcj20160687
摘要

Escherichia coli O157:H7 is a subtype of Shiga toxin-producing E. coli that is associated with haemorrhagic colitis and haemolytic uremic syndrome (HUS). Studies of populations in endemic areas have reported that the presence of specific antibodies against the O157 lipopolysaccharide (LPS) is associated with a lower incidence of diarrhoea and HUS. Phage display and IgG anti-O157 LPS antibodies were used in the present study to select peptide mimotopes of O157 LPS expressed in protein III of the M13 phage. Synthetic peptides (SP) were designed using the derived amino acid sequences obtained from DNA nucleotides of 63 selected phagotopes. The LxP/YP/SxL motif was identified in five of the phagotope amino acid sequences. Antibody responses against the phagotopes and their corresponding SPs were evaluated. SP12, one of the designed SP, induced the production of antibodies against the homologous peptide (1:800) and O157 LPS (1:200). The specificity of anti-SP12 antiserum was confirmed by analyzing its response to SP3, an SP with a different amino acid sequence than that of SP12, as well as against an E. coli LPS different from O157. Competitive studies with SP12 and O157 LPS showed a significant decrease in anti-SP12 and anti-LPS O157 antiserum responses against SP12 and O157 LPS, respectively. Eighteen (82%) of the 22 human serum samples with positive reactivity against E coli O157 LPS reacted with SP12 SP (cut-off >0.4). These results support the idea that SP12 is an immunogenic mimotope of O157 LPS.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
kenna123发布了新的文献求助10
刚刚
颖中竹子完成签到,获得积分10
1秒前
小宫完成签到,获得积分20
1秒前
3秒前
4秒前
4秒前
鸭鸭发布了新的文献求助20
4秒前
豆子完成签到 ,获得积分10
6秒前
7秒前
ely发布了新的文献求助10
7秒前
7秒前
潇洒的凝冬完成签到,获得积分10
8秒前
李健的小迷弟应助kenna123采纳,获得10
8秒前
纸飞机发布了新的文献求助10
9秒前
小蘑菇应助洗剪吹采纳,获得10
11秒前
haowu发布了新的文献求助10
12秒前
无心的书发布了新的文献求助10
13秒前
飘落发布了新的文献求助10
14秒前
Survivor完成签到,获得积分10
14秒前
林铮完成签到,获得积分10
17秒前
Eric完成签到,获得积分10
19秒前
yan发布了新的文献求助10
20秒前
CurryFan发布了新的文献求助20
21秒前
炙热靖雁发布了新的文献求助30
23秒前
平常的傲白完成签到,获得积分10
25秒前
哈比人linling完成签到,获得积分10
26秒前
vision0000完成签到,获得积分10
29秒前
30秒前
32秒前
yrr发布了新的文献求助10
33秒前
oceanao应助科研通管家采纳,获得10
34秒前
几又应助科研通管家采纳,获得10
34秒前
852应助科研通管家采纳,获得10
34秒前
科研通AI2S应助科研通管家采纳,获得10
34秒前
大模型应助科研通管家采纳,获得10
34秒前
科研通AI2S应助科研通管家采纳,获得10
34秒前
CodeCraft应助科研通管家采纳,获得10
34秒前
MoonFlows应助科研通管家采纳,获得20
34秒前
34秒前
高分求助中
Evolution 10000
Becoming: An Introduction to Jung's Concept of Individuation 600
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
The Kinetic Nitration and Basicity of 1,2,4-Triazol-5-ones 440
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3164126
求助须知:如何正确求助?哪些是违规求助? 2814837
关于积分的说明 7906792
捐赠科研通 2474446
什么是DOI,文献DOI怎么找? 1317493
科研通“疑难数据库(出版商)”最低求助积分说明 631818
版权声明 602228