兰克尔
破骨细胞
化学
成骨细胞
双酚
细胞凋亡
细胞生物学
MAPK/ERK通路
蛋白激酶B
细胞分化
内科学
内分泌学
体外
激酶
生物
生物化学
受体
医学
环氧树脂
有机化学
基因
激活剂(遗传学)
作者
Jin Kyung Hwang,Kwan Hee Min,Kyoung Hee Choi,You‐Cheol Hwang,In‐Kyung Jeong,Kyu Jeung Ahn,Ho-Yeon Chung,Jae Suk Chang
出处
期刊:Life Sciences
[Elsevier]
日期:2013-09-01
卷期号:93 (9-11): 367-372
被引量:80
标识
DOI:10.1016/j.lfs.2013.07.020
摘要
Bisphenol A (BPA), a major component of epoxy resins used in protective coatings, is a known endocrine-disrupting chemical. BPA has the ability of binding to estrogen receptors. In the current paper, we examine the direct effects of bisphenol A on in vitro osteoclast and osteoblast culture systems. We evaluated the effects of BPA on osteoclast formation using bone marrow-derived macrophages and RAW 264.7 cells and on osteoblast differentiation using MC3T3-E1 cells. BPA significantly inhibited RANKL-induced, TRAP-positive multinucleated cell formation in bone marrow-derived macrophages and RAW 264.7 cell cultures in a dose-dependent manner (0.5 μM to 12.5 μM). We observed suppression of ERK, JNK, AKT, and p38 mitogen-activated protein kinases induced by RANKL in Western blotting after BPA treatment in RAW 264.7 cells. Furthermore, BPA suppressed Bcl-2 (anti-apoptotic) while stimulating Bax (pro-apoptotic) protein expression in RAW 264.7 cells. Bisphenol A also significantly suppressed ALP activities and bone nodule formation in MC3T3-E1 cell cultures. Specifically, the expression of Bcl-2 protein was decreased, and changes in expression of caspases 3, 8, and 9 were detected by BPA treatment in both cells. We found that bisphenol A directly suppressed both osteoclastic and osteoblastic activities in vitro. Our data suggest that bisphenol A suppresses cell differentiation and survival.
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