Osteocyte-derived exosomes inhibit osteoblast activity and induce osteoclast formation

骨细胞 微泡 破骨细胞 成骨细胞 兰克尔 细胞生物学 旁分泌信号 化学 生物 生物化学 体外 小RNA 受体 激活剂(遗传学) 基因
作者
Xiaodi Sun,Yanying Wang,Ke Gu,Qingfu Wang,Jing Yang,Yupu Mao,Huanze Deng,Jian Zhang
出处
期刊:Materials Express [American Scientific Publishers]
卷期号:11 (1): 46-53 被引量:3
标识
DOI:10.1166/mex.2021.1887
摘要

Osteocytes, which are the most abundant cell type in bone, regulate osteoblasts and osteoclasts via both cell–cell interactions and paracrine signaling, and osteocyte-derived exosomes might contribute to this paracrine action. In this study, we investigated the effects of osteocyte-derived exosomes on regulating osteoblasts and osteoclasts and studied the potential mechanism. Materials and Methods : Osteocyte-derived exosomes were extracted and identified. PKH67-labeled exosomes were incubated with MC3T3-E1 cells and RAW264.7 cells, and fluorescence confocal microscopy was used to analyze the uptake of exosomes. ALP stain- ing and TRAP staining were used to analyze osteoblast activity and osteoclast formation. The level of miR-214-3p in exosomes was analyzed by qPCR and the incorporation of FAM-labeled miR-214-3p from exosomes into MC3T3-E1 cells was evaluated. The expressions of ephrinA2 and RANKL in exosomes were studied. Results : Our results demonstrated that osteocyte-derived exosomes might recognize osteoblasts through the ephrinA2 protein; thus, miR-214-3p in exosomes was transferred into osteoblasts to inhibit osteoblast activity. Meanwhile, we found that osteocyte-derived exosomes could be transferred into osteoclasts to induce osteoclast formation by releasing RANKL. Conclusion : These findings suggest that osteocyte-derived exosomes play an important role in the regulation of osteoblast and osteoclast activity, which might occur via miR-214-3p and RANKL.
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