Determination of ursolic acid in ethanolic extract of Rotheca serrata (L.) Steane & Mabb. by HPLC method

(L.) Steane & Mabb. a plant having significant medicinal value is used in treating a fever, rheumatism, inflammation, pain, and malarial fever. Ursolic acid has a five-ringed pentacyclic structure belongs to the family of triterpenoids. It has antioxidant, antitumor, and hepato-protective properties. The method in HPLC was developed on a SHIMADZU, Shimpack ODS (25 cm × 4.6 mm, 5 µm) column. The mobile phase consisted of a 70:25:5 (v/v/v) mixture of methanol, acetonitrile, and 0.02 M ammonium acetate buffer (pH 3.5). A flow rate of 1.2 mL/min and λ