Characterization of cellulases from softening fruit for enzymatic depolymerization of cellulose

解聚 纤维素酶 纤维素 软化 表征(材料科学) 化学 酶水解 高分子科学 有机化学 生物化学 高分子化学 材料科学 复合材料 纳米技术
作者
Hilary Edema,Muhammad Furqan Ashraf,Amos Samkumar,Laura Jaakola,Katja Karppinen
出处
期刊:Carbohydrate Polymers [Elsevier]
卷期号:343: 122493-122493 被引量:1
标识
DOI:10.1016/j.carbpol.2024.122493
摘要

Cellulose is a major renewable resource for a wide variety of sustainable industrial products. However, for its utilization, finding new efficient enzymes for plant cell wall depolymerization is crucial. In addition to microbial sources, cellulases also exist in plants, however, are less studied. Fleshy fruit ripening includes enzymatic cell wall hydrolysis, leading to tissue softening. Therefore, bilberry (Vaccinium myrtillus L.), which produces small fruits that undergo extensive and rapid softening, was selected to explore cellulases of plant origin. We identified 20 glycoside hydrolase family 9 (GH9) cellulases from a recently sequenced bilberry genome, including four of which showed fruit ripening-specific expression and could be associated with fruit softening based on phylogenetic, transcriptomic and gene expression analyses. These four cellulases were secreted enzymes: two B-types and two C-types with a carbohydrate binding module 49. For functional characterization, these four cellulases were expressed in Pichia pastoris. All recombinant enzymes demonstrated glucanase activity toward cellulose and hemicellulose substrates. Particularly, VmGH9C1 demonstrated high activity and ability to degrade cellulose, xyloglucan, and glucomannan. In addition, all the enzymes retained activity under wide pH (6-10) and temperature ranges (optimum 70 °C), revealing the potential applications of plant GH9 cellulases in the industrial bioprocessing of lignocellulose.
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