促炎细胞因子
鼻息肉
适应(眼睛)
背景(考古学)
化学
炎症
细胞生物学
生物
医学
癌症研究
内科学
神经科学
古生物学
作者
Hao Chen,Qianqian Zhang,Jiani Chen,Han Li,Fuying Cheng,Yizhang Wang,Yingqi Gao,Yumin Zhou,Le Shi,Yufei Yang,Бо Лю,Kai Xue,Yaguang Zhang,Hongmeng Yu,Sheng Wang,Li Hu,Li Wang,Kai Xue
出处
期刊:JCI insight
[American Society for Clinical Investigation]
日期:2024-10-03
标识
DOI:10.1172/jci.insight.184739
摘要
Chronic rhinosinusitis with nasal polyps (CRSwNP) is an inflammatory upper airway disease, divided into eosinophilic CRSwNP (eCRSwNP) and noneosinophilic CRSwNP (neCRSwNP) according to eosinophilic levels. Neutrophils are major effector cells in CRSwNP. but their role in different inflammatory environments remain largely unclear. We performed an integrated transcriptome analysis of polyp-infiltrating neutrophils from CRSwNP patients, using healthy donor blood as a control. Flow cytometry and in vitro studies showed that neutrophils are activated in both CRSwNP type. The scRNA-sequencing analysis demonstrated that neutrophils were classified into five functional subsets, with GBP5+ neutrophils occurring mainly in neCRSwNPs and a high proportion of CXCL8+ neutrophils in both subendotypes. GBP5+ neutrophils exhibited significant IFN-I pathway activity in neCRSwNPs. CXCL8+ neutrophils displayed increased neutrophil activation scores and mainly secrete Oncostatin M (OSM), which facilitates communication with other cells. In vitro experiments revealed that OSM could enhance IL-13- or IL-17-mediated immune responses in nasal epithelial cells and fibroblasts. Our findings revealed that neutrophils exhibited transcriptional plasticity and activation when exposed to polyp tissue and exert their proinflammatory role in the pathogenesis of CRSwNP by releasing OSM to interact with epithelial cells and fibroblasts in a manner dependent on the inflammatory milieu.
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