Integrated Analysis of Vietnamese Illigera trifoliata ssp. cucullata (Merr.) Kubitzki), Leaf and Stem Essential Oils by GC-FID/GC-MS and 13C NMR

Illigera trifoliata ssp.cucullata (Merr.)Kubitzki (Hernandiaceae) is a liana distributed mainly in the tropical regions of Asia.Nothing is known on the phytochemicals produced by this species.Oil samples have been isolated from leaves, and stems, and analyzed by combination of chromatographic and spectroscopic techniques [GC(FID), GC-MS and 13 C NMR].The compositions of leaf and stem oils were dominated by phellandrene (25.8 and 29.2%), -pinene (26.0 and 9.5%) and -phellandrene (12.8 and 15.8%), followed by limonene (5.5 and 8.1%) and -pinene (7.5 and 3.0%).The stem essential oil from Illigera trifoliata ssp.cucullata exhibited antibacterial activity against Bacillus subtilis and Escherichia coli with MIC values of 4000 μg/mL and IC50 values of 1824,56 ±136 and 2666,66 ±257 μg/mL, respectively.