Use of Micrografting to Study the Role Played by Peptide Signals in ABA Biosynthesis

Plants survive by deploying appropriate stress responses under rapidly changing environmental conditions. Vascular plants transmit environmental information among distant organs. Long-distance signaling plays a crucial role in plant adaptation and subsequent survival under severe environmental conditions. In model plants, the micrografting method has recently emerged as an important method to elucidate tissue-to-tissue communication via hormones, RNAs, and peptides. In this chapter, I describe a micrografting method for Arabidopsis thaliana to graft shoots onto roots of plants with different genotypes. This grafting method may facilitate further research investigating how vascular plants integrate environmental information among distant organs via long-distance signaling.