蛋白质工程
合成生物学
计算生物学
细胞生物学
基因工程
生物
化学
生物化学
基因
酶
作者
Guihua Zeng,Huanqiu Li,Yongyi Wei,Weimin Xuan,Roushu Zhang,Larisa E. Breden,Wei Wang,Fu‐Sen Liang
标识
DOI:10.1021/acssynbio.6b00255
摘要
A new synthetic biology engineering strategy integrating chemical reactivity sensing and small molecule induced protein dimerization has been developed to generate artificial Fe2+ signaling circuitry to control tailored cellular events in mammalian cells. The dual function probe ABA-FE18 (Fe2+-sensing and protein dimerization) derived from ABA was developed and used to control gene activation, signal transduction, and cytoskeletal remodeling in response to Fe2+. This technology was utilized to design signal circuitry incorporating "AND" and "OR" biologic gates that enables mammalian cells to translate different combinations of Fe2+ and H2O2 signals into predefined biological outputs.
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