角质层
毛囊
渗透(战争)
氰基丙烯酸酯
无毛
非那雄胺
剥离(纤维)
化学
色谱法
生物医学工程
皮肤病科
材料科学
病理
医学
内分泌学
内科学
数学
生物化学
前列腺
胶粘剂
有机化学
运筹学
图层(电子)
癌症
复合材料
作者
Silvia Tampucci,Susi Burgalassi,Patrizia Chetoni,Carla Lenzi,Andrea Pirone,F. Mailland,Maurizio Caserini,Daniela Monti
摘要
The differential stripping technique consists of a tape-stripping phase followed by a cyanoacrylate biopsy. This technique not only allows the quantification of drug retained in the stratum corneum (SC) and in the hair follicles but also differentiates transepidermal from transfollicular penetration. Our study aimed at both validating the differential stripping procedure on hairless rat skin and assessing the role of the hair follicle in the cutaneous penetration of finasteride (FNS) after application of two experimental formulations for 6 or 24 h: P-08–016, a hydroxypropyl chitosan (HPCH)-based formulation and P-10–008, an anhydrous formulation devoid of HPCH. Microscopic and histological evaluation showed that after 15 tape strips both the SC and the viable epidermis were completely removed. A subsequent cyanoacrylate skin surface biopsy led to the removal of the infundibula content. The largest amounts of FNS were found in the epidermis and in the appendages after application of P-08–016, regardless of the time from application. In contrast, smaller and statistically significant amounts of FNS were recovered with P-10–008 6 h after application, compared with that at 24 h. In conclusion, the differential stripping technique allowed determination of the amount of FNS localized in different skin districts, focusing particularly on the follicular contribution. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:2323–2329, 2014
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