生物膜
铜
温度梯度凝胶电泳
生物污染
甲基丙烯酸甲酯
材料科学
环境科学
化学
复合材料
细菌
生物
聚合物
冶金
共聚物
生物化学
遗传学
16S核糖体RNA
膜
作者
Alexandra Meier,Nefeli-Maria Tsaloglou,Matthew C. Mowlem,C. W. Keevil,Douglas P. Connelly
出处
期刊:Biofouling
[Informa]
日期:2013-08-22
卷期号:29 (9): 1029-1042
被引量:18
标识
DOI:10.1080/08927014.2013.824967
摘要
Biofouling is a major problem for long-term deployment of sensors in the marine environment. This study showed that significant biofilm formation occurred on a variety of artificial materials (glass, copper, Delrin(™) and poly-methyl methacrylate [PMMA]) deployed for 10 days at a depth of 4700 m in the Cayman Trough. Biofilm surface coverage was used as an indicator of biomass. The lowest biofilm coverage was on copper and PMMA. Molecular analyses indicated that bacteria dominated the biofilms found on copper, Delrin(™) and PMMA with 75, 55 and 73% coverage, respectively. Archea (66%) were dominant on the glass surface simulating interior sensor conditions, whereas Eukarya comprised the highest percentage of microflora (75%) on the glass simulating the exterior of sensors. Analysis of Denaturing Gradient Gel Electrophoresis profiles indicated that copper and Delrin(™) shared the same community diversity, which was not the case for glass and PMMA, or between PMMA and copper/Delrin(™). Sequence alignment matches belonged exclusively to uncultivable microorganisms, most of which were not further classified. One extracted sequence found on glass was associated with Cowellia sp., while another extracted from the PMMA surface was associated with a bacterium in the Alterominidaceae, both γ-proteobacteria. The results demonstrate the necessity of understanding biofilm formation in the deep sea and the potential need for mitigation strategies for any kind of long-term deployment of remote sensors in the marine environment.
科研通智能强力驱动
Strongly Powered by AbleSci AI