化学
酵母
蛋白质-蛋白质相互作用
酿酒酵母
泛素
细胞生物学
膜蛋白
双杂交筛选
泛素连接酶
跨膜蛋白
生物化学
整体膜蛋白
作者
Logan Bashline,Ying Gu
出处
期刊:Methods in molecular biology
日期:2014-10-30
卷期号:: 143-158
被引量:4
标识
DOI:10.1007/978-1-4939-1902-4_13
摘要
Proteins are responsible for many biological processes within living organisms. Many proteins have the ability to specifically interact with other proteins in order to function properly. The identification of protein–protein interactions (PPIs) can provide useful information about the function of a protein of interest. Historically, the properties of transmembrane proteins have caused difficulty in analyzing PPIs among transmembrane proteins. The development of an assay that is capable of analyzing PPIs involving transmembrane proteins, the split-ubiquitin yeast two-hybrid (SU-Y2H) assay, has provided a method to probe pairwise PPIs between two proteins of interest or to screen a single protein of interest for interaction partners. The following protocol explains how to use the SU-Y2H assay, which is compatible with the use of transmembrane proteins, to investigate PPIs between two proteins of interest and also briefly describes how to adjust the system to be used as a high-throughput screen for interaction partners of a particular protein of interest.
科研通智能强力驱动
Strongly Powered by AbleSci AI