Application of Bioluminescence Resonance Energy Transfer (BRET) for Biomolecular Interaction Studies

Bioluminescence in BIA. Bioluminescence resonance energy transfer (BRET) can be utilised for biomolecular interaction analysis (BIA) to quantify protein–protein interactions in living cells. This was demonstrated with the cAMP-dependent protein kinase regulatory and catalytic subunits fused to Renilla luciferase (Rluc) and GFP2, respectively.