Marixanthotalea marina gen. nov., sp. nov., a bacterium in the family Flavobacteriaceae isolated from seawater

A Gram-stain-negative, yellow-pigmented, non-motile, rod-shaped, catalase-positive, strictly aerobic marine bacterium, designated XHP0103 T , was isolated from seawater collected from the southern Yellow Sea, PR China (34° 45′ 53″ N 119° 25′ 30″ E). Strain XHP0103 T grew optimally at 28 °C, pH 7.5 and in 1.0–3.0 % (w/v) sea salt. MK-6 was the major respiratory quinone. The major cellular fatty acids (>10%) were iso-C 15 : 0 , iso-C 15 : 1 G and iso-C 17 : 0 3-OH. The polar lipid profile contained phosphatidylethanolamine, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. Results of 16S rRNA gene sequence analysis indicated that strain XHP0103 T displayed highest sequence similarity to Aestuariibaculum marinum IP7 T (94.1 %). However, the phylogenetic trees based on 16S rRNA gene sequences suggested that strain XHP0103 T clustered with Tamlana crocina HST1-43 T (93.4 % sequence similarity) and Aestuariivivens insulae AH-MY3 T (93.5 %). Genome sequencing revealed that strain XHP0103 T comprised 3 134 388 bp with 2770 protein-coding genes, and the DNA G+C content was 35.5 %. The average nucleotide identity and digital DNA–DNA hybridization values between strain XHP0103 T and T. crocina HST1-43 T were 73.6 and 17.3 %, respectively. Based on phylogenetic, phenotypic, genomic and chemotaxonomic evidence, strain XHP0103 T represents a novel genus in the family Flavobacteriaceae , for which the name Marixanthotalea marina gen. nov., sp. nov. is proposed. The type strain is XHP0103 T (=MCCC 1K06060 T =JCM 34682 T ).