亲和层析
单克隆抗体
化学
抗体
色谱法
蛋白质A
产量(工程)
串联亲和纯化
蛋白质纯化
生物化学
生物
酶
材料科学
免疫学
冶金
作者
Anurag S. Rathore,Sharad Narnaware
出处
期刊:Methods in molecular biology
日期:2021-09-03
卷期号:: 169-177
被引量:8
标识
DOI:10.1007/978-1-0716-1450-1_9
摘要
Protein A affinity chromatography is widely used for capturing therapeutic antibodies. It offers high binding capacity, selectivity, and resin reusability while delivering high yield and product purity. The Protein A step is ubiquitous in its presence in purification platforms for production of antibody products due to the effective clearance it offers of impurities, high and low molecular weight species (HMW and LMW), host cell proteins (HCP), and DNA. In this chapter, we describe a highly selective Protein A affinity chromatography protocol for purification of monoclonal antibodies.
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