染色质
染色质免疫沉淀
组蛋白
计算生物学
芯片对芯片
细胞生物学
DNA
生物
抄写(语言学)
基因
基因表达
遗传学
染色质重塑
发起人
语言学
哲学
作者
Weizhi Ouyang,Xingwang Li
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 109-117
标识
DOI:10.1007/978-1-0716-3354-0_8
摘要
Histone post-translational modifications and transcription factors (TFs) play vital roles in regulating gene expression. A comprehensive understanding of transcriptional regulation requires genome-wide mapping of chromatin features such as histone modifications and TF binding sites. Here, we describe a detailed nucleus CUT&Tag (Cleavage Under Targets and Tagmentation) protocol, which is an antibody-guided in situ protein-DNA interaction mapping method using protein A/G fused Tn5 transposase. Compared with regular ChIP-seq in plants, nucleus CUT&Tag (nCUT&Tag) omits many steps such as sonication and immunoprecipitation, thus saving much time and making it possible to efficiently profile chromatin features from low-input and even single cells with higher signal-to-noise ratio.
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