粘液纤毛清除率
呼吸上皮
气道
纤毛
上皮
肥胖
医学
功能(生物学)
细胞生物学
内分泌学
生物
内科学
肺
病理
麻醉
作者
Yuko Tanaka,Tomoyuki Fujisawa,Shusuke Yazawa,Isao Ohta,Yasuharu Takaku,Masahiko Ito,Yusuke Inoue,Hideki Yasui,Hironao Hozumi,Masato Karayama,Yuzo Suzuki,Kazuki Furuhashi,Noriyuki Enomoto,Mitsutoshi Setou,Naoki Inui,Tetsuro Suzuki,Takafumi Suda
出处
期刊:American Journal of Physiology-lung Cellular and Molecular Physiology
[American Physiological Society]
日期:2024-08-06
卷期号:327 (3): L406-L414
标识
DOI:10.1152/ajplung.00114.2024
摘要
Obesity is a risk factor for increased morbidity and mortality in viral respiratory infection. Mucociliary clearance (MCC) in the airway is the primary host defense against viral infections. However, the impact of obesity on MCC is unclear, prompting this study. Using murine tracheal tissue culture and in vitro influenza A virus (IAV) infection models, we analyzed cilia-driven flow and ciliary beat frequency (CBF) in the airway epithelium to evaluate MCC. Short-term IAV infection increased cilia-driven flow and CBF in control mice, but not in high-fat diet-induced obese mice. Basal cilia-driven flow and CBF were also lower in obese mice than in control mice. Mechanistically, the increase of extracellular adenosine triphosphate (ATP) release during IAV infection, which was observed in the control mice, was abolished in the obese mice; however, the addition of ATP increased cilia-driven flow and CBF both in control and obese mice to a similar extent. In addition, RNA sequencing and reverse transcription-polymerase chain reaction revealed the downregulation of several cilia-related genes, including
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