Real-time and programmable transcriptome sequencing with PROFIT-seq v1

PROgrammable Full-length Isoform Transcriptome sequencing (PROFIT-seq) is a method that enriches target transcripts while maintaining unbiased quantification of the whole transcriptome. PROFIT-seq employs combinatorial reverse transcription to capture polyadenylated, non-polyadenylated, and circular RNAs, coupled with nanopore adaptive sampling that selectively enriches target transcripts during sequencing.