肺表面活性物质
响应面法
色谱法
分散性
化学
中心组合设计
成分
乳状液
萃取(化学)
山茶
分式析因设计
超临界流体
超临界流体萃取
化学工程
析因实验
食品科学
有机化学
植物
生物化学
统计
数学
生物
工程类
作者
Nara Yaowiwat,Worrapan Poomanee,Pimporn Leelapornpisid,Phanuphong Chaiwut
出处
期刊:ACS omega
[American Chemical Society]
日期:2023-07-26
卷期号:8 (31): 28090-28097
标识
DOI:10.1021/acsomega.3c00602
摘要
This study aimed to obtain tea flower extract (TFE) using supercritical fluid extraction, to determine the compounds present in the TFE and to establish its antioxidant activity. The fabrication of TFE nanoemulsions was also investigated using response surface methodology (RSM). UHPLC-ESI-QTOF-MS/MS and UHPLC-ESI-QqQ-MS/MS analysis showed that the TFE was composed of catechin and its derivatives, flavonols and anthocyanins, suggesting its potential as a free radical scavenger with strong reducing powers. A central composite design was applied to optimize the independent factors of the nanoemulsions. The factors had a significant (p < 0.05) effect on all response variables. The optimum level of factors for the fabrication was a surfactant-to-oil ratio of 2:1, a high hydrophilic-lipophilic balance (HLB) surfactant to low HLB surfactant ratio (HLR) of 1.6:1, and a PEG-40/PEG-60 hydrogenated castor oil ratio of 2:1. The responses obtained from the optimum levels were a 34.01 nm droplet size, a polydispersity index of 0.15, and 75.85% entrapment efficiency. In conclusion, TFE could be an antioxidant active ingredient and has been successfully loaded into nanoemulsions using RSM.
科研通智能强力驱动
Strongly Powered by AbleSci AI