Genetic dissection of resistance to gray leaf spot by genome-wide association study in a multi-parent maize population

生物 候选基因 遗传学 全基因组关联研究 单核苷酸多态性 遗传关联 基因分型 数量性状位点 基因 人口 单倍型 生物技术 基因型 人口学 社会学
作者
Can Hu,Tianhui Kuang,Ranjan K. Shaw,Yudong Zhang,Jun Fan,Yaqi Bi,Fuyan Jiang,Ruijia Guo,Xingming Fan
出处
期刊:BMC Plant Biology [Springer Nature]
卷期号:24 (1) 被引量:3
标识
DOI:10.1186/s12870-023-04701-1
摘要

Abstract Background Understanding the genetic mechanisms underlying gray leaf spot (GLS) resistance in maize is crucial for breeding GLS-resistant inbred lines and commercial hybrids. Genome-wide association studies (GWAS) and gene functional annotation are valuable methods for identifying potential SNPs (single nucleotide polymorphism) and candidate genes associated with GLS resistance in maize. Results In this study, a total of 757 lines from five recombinant inbred line (RIL) populations of maize at the F 7 generation were used to construct an association mapping panel. SNPs obtained through genotyping-by-sequencing (GBS) were used to perform GWAS for GLS resistance using a linear mixture model in GEMMA. Candidate gene screening was performed by analyzing the 10 kb region upstream and downstream of the significantly associated SNPs linked to GLS resistance. Through GWAS analysis of multi-location phenotypic data, we identified ten candidate genes that were consistently detected in two locations or from one location along with best linear unbiased estimates (BLUE). One of these candidate genes, Zm00001d003257 that might impact GLS resistance by regulating gibberellin content, was further identified through haplotype-based association analysis, candidate gene expression analysis, and previous reports. Conclusions The discovery of the novel candidate gene provides valuable genomic resources for elucidating the genetic mechanisms underlying GLS resistance in maize. Additionally, these findings will contribute to the development of new genetic resources by utilizing molecular markers to facilitate the genetic improvement and breeding of maize for GLS resistance.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
星辰予卓完成签到 ,获得积分10
1秒前
Elysia完成签到,获得积分10
1秒前
堂风完成签到,获得积分10
1秒前
1秒前
庾磬完成签到,获得积分10
3秒前
海天完成签到 ,获得积分10
3秒前
4秒前
星辰予卓关注了科研通微信公众号
5秒前
多比完成签到 ,获得积分10
6秒前
小酒迟疑发布了新的文献求助10
7秒前
乱七八糟完成签到 ,获得积分10
8秒前
11秒前
NexusExplorer应助书起洛阳采纳,获得10
11秒前
YanZhe完成签到,获得积分10
11秒前
永不停歇奈格里完成签到,获得积分10
15秒前
wenwen完成签到 ,获得积分10
16秒前
科研通AI2S应助茶博士采纳,获得10
17秒前
wuhao0118发布了新的文献求助10
17秒前
17秒前
19秒前
19秒前
张张完成签到 ,获得积分10
20秒前
20秒前
三顿饭吃一天完成签到,获得积分10
21秒前
22秒前
jackguihx完成签到,获得积分20
22秒前
susuna完成签到,获得积分10
22秒前
LJT发布了新的文献求助10
23秒前
wuhao0118完成签到,获得积分10
24秒前
realrrr发布了新的文献求助10
24秒前
田様应助小酒迟疑采纳,获得10
26秒前
26秒前
丘比特应助科研通管家采纳,获得10
28秒前
科研通AI2S应助科研通管家采纳,获得10
28秒前
CodeCraft应助科研通管家采纳,获得10
28秒前
科研通AI2S应助科研通管家采纳,获得10
28秒前
鲍文启完成签到 ,获得积分10
28秒前
孤鹰完成签到 ,获得积分10
29秒前
书起洛阳发布了新的文献求助10
31秒前
毛豆应助包佳梁采纳,获得10
32秒前
高分求助中
Sustainability in ’Tides Chemistry 1500
The ACS Guide to Scholarly Communication 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Handbook of the Mammals of the World – Volume 3: Primates 805
Ethnicities: Media, Health, and Coping 800
Historia de la ciencia jurídica europea 600
Gerard de Lairesse : an artist between stage and studio 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3069575
求助须知:如何正确求助?哪些是违规求助? 2723483
关于积分的说明 7481948
捐赠科研通 2370550
什么是DOI,文献DOI怎么找? 1257057
科研通“疑难数据库(出版商)”最低求助积分说明 609800
版权声明 596861