软骨发生
细胞生物学
MAPK/ERK通路
间质细胞
化学
间充质干细胞
归巢(生物学)
蛋白激酶B
干细胞
激酶
生物
信号转导
癌症研究
生态学
作者
Yiming Wang,Jifei Chen,Fan Yan Wei,Jing Zhang,Bingxuan Hua,Bolin Sun,Liang Zhu,Xinhao Niu,Zuoqin Yan,Changan Guo
出处
期刊:PubMed
日期:2017-01-01
卷期号:9 (5): 2656-2667
被引量:4
摘要
The clinical translation of tissue engineering methods is confined by the limited external cell sources, which is hopefully to be addressed by the cell guidance approach as cytokine-induced homing and differentiation of the patients' autologous cells. Synovium-derived stem cells (SDSCs) are a potent cell source for cartilage restoration due to its intrinsic proximity and tissue-specific chondrogenic capacity. In this study, stromal cell-derived factor-1α (SDF-1α) in combination with transforming growth factor β1 (TGF-β1) were used to induce SDSCs migration and chondrogenesis in vitro. The migration capacity was evaluated by transwell assay and for chondrogenic evaluation, the expression of Sox9, ACAN and COL2A1 were assessed by quantitative RT-PCR while the expression of sulfated GAG and collagen II were evaluated by Alcian Blue stain and immunohistochemistry respectively. Our data showed that SDF-1α/CXC chemokine receptor 4 (CXCR4) was involved in SDSCs migration through phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway. Exogenous TGF-β1 enhanced SDF-1α-induced SDSCs migration in a concentration and time-dependent manner through CXCR4, evidenced as complete blockage by AMD3100, the CXCR4 antagonist and this effect was mediated by extracellular regulated protein kinases (ERK) activation. Moreover, the addition of SDF-1α augmented the TGF-β1-induced SDSCs chondrogenesis, evidenced by the increased pellet sizes and the expressions of COL 2A1, ACAN and Sox9. This effect was related to c-Jun N-terminal kinase (JNK) activation. Collectively, these results suggest that SDF-1α and TGF-β1 interacts with each other and synergistically enhance the SDSCs migration and chondrogenesis through MAPK pathways.
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