Analysis of synthetic cannabinoids in wastewater of major cities in China

Synthetic cannabinoids (SCs) could pose serious health risks to its users. It is necessary to monitor its community consumption. Wastewater-based epidemiology is a potentially useful approach in this regard. However, limited research has been conducted to investigate the occurrence of SCs in wastewater. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was optimized to analyze 8 SCs and metabolites (in total 16 analytes) in wastewater. The limit of quantification for this method for certain analytes in wastewater was as low as 0.03 ng L-1. The validated method was used to examine the stability of the analytes under different conditions and to examine their occurrence in wastewater collected from 31 major cities across China. The overwhelming majority of the analytes were stable within 24 h, even at room temperature. However, 5-fluoro MDMB-PICA and MDMB-4en-PINACA butanoic acid metabolite showed significant degradation within 120 days even when stored at -20 °C or -80 °C. At least one cannabinoid or their metabolite was detected in 21 cities. In the city with the highest detection rate, at least one synthetic cannabinoid or metabolite was detected in 95% of samples of the city. MDMB-4en-PINACA butanoic acid metabolite had the highest detection frequency (in 13.4% of the samples). These results indicated that SCs were used in a significant number of Chinese cities. A few parent drugs (MDMB-4en-PINACA, ADB-BUTINACA, 5-fluoro MDMB-PICA, 4-fluoro MDMB-BUTINACA) were detected in a small fraction of wastewater samples, possibly due to release from manufacturing of these cannabinoids or illegal addition of electronic cigarettes.