过敏原
免疫球蛋白E
组胺
化学
花粉
敏化
免疫学
嗜碱性粒细胞
嗜碱性粒细胞活化
重组DNA
表位
过敏
体外
生物化学
药理学
医学
抗体
生物
植物
基因
作者
Andreas Hoffmann,Agnès Jamin,Kay Foetisch,S. May,H Aulepp,Dieter Haustein,Stefan Vieths
出处
期刊:Allergy
[Wiley]
日期:1999-05-01
卷期号:54 (5): 446-454
被引量:60
标识
DOI:10.1034/j.1398-9995.1999.00917.x
摘要
Background: A murine in vitro model of the allergic type I reaction was set up to determine the biologic activity of extracts without involvement of human beings. It is based on β‐hexosaminidase release from passively sensitized RBL cells after allergen challenge. The intended application of this RBL cell assay in the field of quality control of allergenic extracts requires its comparison with established methods. Methods: The activity of five standardized birch‐pollen prick test solutions was determined in parallel by RBL assay, direct IgE binding, IgE‐binding inhibition, major allergen content, histamine‐release assay, and skin testing. Results: The RBL cell‐release assay corresponded well to other methods if a reagin raised against natural birch‐pollen extract was used for passive sensitization. However, in the case of a reagin against recombinant Bet v 1, only a decreased activity was observed, presumably because a reduced number of epitopes were recognized by the monospecific reagin. In contrast to standardized birch‐pollen extracts, nonstandardized apple extracts showed poor activity in all assays. Conclusions: This murine model might be a useful tool in the quality control of allergenic extracts. It combines properties of assays based on standardized antisera and of assays that consider IgE cross‐linking properties.
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