链脲佐菌素
神经保护
自噬
海马体
亚甲蓝
药理学
脂多糖
医学
化学
内科学
内分泌学
生物化学
细胞凋亡
糖尿病
光催化
催化作用
作者
E. V. Stelmashook,Д. Н. Воронков,А. V. Stavrovskaya,Svetlana V. Novikova,Nina G. Yamshikova,Artem S. Olshanskij,Anastasiya S. Guschina,Margarita O. Shedenkova,Е. Е. Генрихс,Н. К. Исаев
出处
期刊:Brain Research
[Elsevier]
日期:2023-04-01
卷期号:1805: 148290-148290
被引量:3
标识
DOI:10.1016/j.brainres.2023.148290
摘要
Methylene blue (MB) can be used as a multidirectional neuroprotector to stop the development of multiple cascades of neuron damage during neurodegenerative processes. This study assesses a protective effect of MB, using an experimental simulation of sporadic Alzheimer's disease by intracerebroventricular administration of streptozotocin (STZ) in rats. It was found that a STZ-induced impairment of memory can be partially mitigated with intravenous injections of MB after the administration of STZ. The treatment of animals with MB prevented the STZ-induced increase in the number and density of microglial and GFAP-positive cells in the brain cortex. In addition, it was shown that the expression of the LC3B protein, an indicator of autophagy, increases in the hippocampus of animals treated with STZ. In the hippocampus of animals treated with MB, an increase in the expression of the LC3B protein was prevented. Using the Griess reaction assay and immunocytochemical study was found that MB reduces lipopolysaccharide-induced NO-production and the expression of iNOS in cultured neurons. In conclusion, our data demonstrate that MB has neuroprotective and anti-inflammatory effects and is able to prevent autophagy. These effects have important therapeutic implications, so MB could potentially play a role in the treatment of neurodegenerative processes.
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