中国仓鼠卵巢细胞
过程开发
效价
水解物
单克隆抗体
化学定义介质
高通量筛选
化学
计算机科学
生物化学
医学
工艺工程
体外
抗体
免疫学
工程类
水解
受体
作者
Qin He,Jianlin Xu,Erik Vandermark,Jun Tian,Yueming Qian,Cynthia Chelius,Jong‐Chan Lee,Michael Borys,Zheng Jian Li,Girish Pendse
出处
期刊:Data in Brief
[Elsevier BV]
日期:2021-10-19
卷期号:39: 107491-107491
被引量:2
标识
DOI:10.1016/j.dib.2021.107491
摘要
In this article, we present four sets of data from high-throughput screening (HTS) studies of different chemically defined media using an industrially relevant Chinese hamster ovary (CHO) cell line. While complex hydrolysate media was used in the early phase process development and manufacturing of a monoclonal antibody (mAb), here we seek to determine an appropriate chemically defined media for late phase process development. Over 150 combinations of chemically defined basal media, feed media, and basal and feed media supplements, such as polyphenolic flavonoid antioxidants (including rosmarinic acid (RA)), were evaluated in four HTS studies to replace the complex hydrolysate media. Specifically, these four screening studies incorporated custom design of experiment (DOE), one-factor-at-a-time (OFAT), and definitive screening design methodologies for titer improvement. Titer was improved two fold compared to the early phase process using the addition of RA to chemically defined media. This dataset exemplifies how HTS can be used as an effective approach to systematically and statistically determine media composition and supplementation to increase mAb titer. These data were presented in connection with a published paper [1].
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