生长素
卡哈尔间质细胞
自噬
细胞凋亡
流式细胞术
内科学
内分泌学
运动性
下调和上调
发病机制
生物
化学
免疫组织化学
医学
免疫学
细胞生物学
生物化学
激素
基因
作者
Yi-Xin Zeng,Li Zhou,Ying Wang,Ting Fu,Pai-Di Xu,Hongxing Zhang,Ying Guan
出处
期刊:Combinatorial Chemistry & High Throughput Screening
[Bentham Science]
日期:2023-10-10
卷期号:26
被引量:2
标识
DOI:10.2174/0113862073262404231004053116
摘要
Functional dyspepsia (FD) is one of the most common gastrointestinal diseases, with a global prevalence of 10%-30%. However, the specific pathogenesis of FD has not yet been determined. As such, the aim of this study was to investigate the effects of saikosaponin D (SSD) administration on the apoptosis, autophagy, and morphological structure of the intestinal cells of Cajal (ICCs) in FD. Methods:A rat model of FD was constructed by stimulating the rat tail with a sponge clamp at one-third of the distal tail length. An autophagy model was constructed for ICCs using glutamate. The apoptosis rate in each group of cells was determined using flow cytometry. The expressions of ghrelin and substance P (SP) were detected using ELISA.The body weight and food intake of male and female rats in the SSD group were consistently higher than those in the model group. The SSD group showed substantial improvement compared with the model group, with no inflammatory cell infiltration and normal gastric mucosal structures. After intervention with SSD, the ultrastructure of the ICCs considerably improved and was clear. Compared with the model group, the expressions of LC3 I/II, ghrelin, and SP proteins in the SSD group were significantly upregulated, and the apoptosis rate was significantly reduced.The administration of SSD improved ICC morphology and structure, inhibited excessive autophagy, and improved FD, a gastrointestinal motility disorder, by regulating ghrelin and SP levels.
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