癌症研究
胰腺癌
下调和上调
小RNA
体内
肿瘤进展
生物
胰腺导管腺癌
原位杂交
体外
上皮-间质转换
癌症
信使核糖核酸
基因
生物技术
生物化学
遗传学
作者
Wen Fu,Xianxing Wang,Ji-Feng Xiang,Shengkai Chen,Renpei Xia,Fanbo Qin,Zhuo Li,Huaizhi Wang,Chuan‐Ming Xie,Changjiang Liu
出处
期刊:Research Square - Research Square
日期:2022-08-05
标识
DOI:10.21203/rs.3.rs-1917234/v1
摘要
Abstract Background Growing evidence suggests that circular RNAs (circRNAs) are important factors in cancer progression. Nevertheless, the role of circRNAs in the progression of pancreatic ductal adenocarcinoma (PDAC) remains unclear. Methods CircPTPRA was identified based on our previous circRNA array data analysis. Wound healing, transwell and EdU assays were performed to investigate the effect of circPTPRA on the migration, invasion and proliferation of PDAC cells in vitro. RNA pull-down, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP), and dual-luciferase reporter assays were conducted to verify the binding of circPTPRA with miR-140-5p. Subcutaneous xenograft models were constructed for in vivo experiments. Results CircPTPRA was significantly upregulated in PDAC tissues and cells compared to normal controls. Moreover, circPTPRA overexpression was positively correlated with lymph node invasion and worse prognosis in PDAC patients. In addition, overexpression of circPTPRA promoted PDAC migration, invasion, proliferation and epithelial-mesenchymal transition (EMT) in vitro and in vivo. Mechanistically, circPTPRA upregulates LaminB1 (LMNB1) expression by sponging miR-140-5p and ultimately promots the progression of PDAC. Conclusions This study revealed that circPTPRA plays an important role in the progression of PDAC by sponging miR-140-5p. It can be explored as a potential prognostic marker and therapeutic target for PDAC.
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