snRNP公司
RNA剪接
生物
拼接因子
SR蛋白
小核核糖核蛋白
核糖核蛋白
外显子剪接增强剂
剪接
小基因
选择性拼接
遗传学
剪接位点突变
细胞生物学
剪接体
RNA结合蛋白
计算生物学
外显子
基因
核糖核酸
作者
Jane Y. Wu,Tom Maniatis
出处
期刊:Cell
[Elsevier]
日期:1993-12-01
卷期号:75 (6): 1061-1070
被引量:737
标识
DOI:10.1016/0092-8674(93)90316-i
摘要
Specific recognition and pairing of the 5′ and 3′ splice sites are critical steps in pre-mRNA splicing. We report that the splicing factors SC35 and specifically interact with both the integral U1 small nuclear ribonucleoprotein (snRNP U1-70K0 and with the 35 kd subunit of the splicing factor U2AF (U2AF35). Previous studies indicated that the U1 snRNP binds specifically to the 5′ splice site, while U2AF35-U2AF35 heterodimer binds to the 3′ splice site. Together, these observations suggest that SC35 and other members of the SR family of splicing factors may function in splice site selection by acting as a bridge between components bound to the 5′ and 3′ splice sites. Interestingly, SC35, , and U2AF35 also interact with the Drosophila splicing regulators Transformer (Tra) and Transformer-2 (Tra2), suggesting that protein-protein interactions mediated by SR proteins may also play an important role in regulating alternative splicing.
科研通智能强力驱动
Strongly Powered by AbleSci AI