Independent chondrogenic potential of canine bone marrow-derived mesenchymal stem cells in monolayer expansion cultures decreases in a passage-dependent pattern

间充质干细胞 软骨发生 化学 细胞生物学 软骨 骨髓 软骨细胞 干细胞 II型胶原 病理 解剖
作者
Eugene C Bwalya,HM Suranji Wijekoon,Jing Fang,Sangho Kim,Kenji Hosoya,Masahiro Okumura
出处
期刊:Journal of Veterinary Medical Science [Japanese Society of Veterinary Science]
卷期号:80 (11): 1681-1687 被引量:1
标识
DOI:10.1292/jvms.18-0202
摘要

Although chondroinductive growth factors are considered necessary for chondrogenesis of bone marrow-derived mesenchymal stem cells (BMSC), independent and spontaneous chondrogenesis has been previously demonstrated in adult horses, bovine calves and adult human BMSC. Surprisingly, adult canine BMSC under similar culture conditions previously failed to demonstrate chondrogenesis. The present study evaluated independent chondrogenic potential of BMSC sourced from three young dogs in the absence of known chondroinductive factors. BMSC were culture expanded in 10% DMEM up to third passage (P3). At each passage, the phenotype of BMSC was evaluated by RT-PCR gel electrophoresis and qPCR. BMSC exhibited a chondrogenic phenotype in the absence of dexamethasone and TGF-β1 as verified by the expression of Sox-9, type II collagen and aggrecan. Sox-9 was significantly downregulated (P<0.05) from P1−P3 compared to P0 while type II and X collagen, and aggrecan were significantly downregulated at P3 compared to P0. There was a significant (P<0.01) negative correlation between passaging and Sox-9, type II collagen and aggrecan gene expression. These results indicate that independent chondrogenic potential and phenotype retention of BMSC decreases in a passage-dependent pattern. Therefore, caution should be exercised for future experiments evaluating the chondrogenic potential of BMSC after extensive expansion cultures in 10% DMEM.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
NexusExplorer应助科研通管家采纳,获得10
刚刚
1秒前
rocky15应助科研通管家采纳,获得200
1秒前
1秒前
田様应助不问归期的风采纳,获得20
1秒前
SciGPT应助科研通管家采纳,获得10
1秒前
传奇3应助科研通管家采纳,获得10
1秒前
积极慕梅应助科研通管家采纳,获得10
1秒前
1秒前
科研通AI2S应助科研通管家采纳,获得10
1秒前
深情安青应助孤独的宛采纳,获得30
1秒前
DRShan发布了新的文献求助10
1秒前
1秒前
2秒前
2秒前
3秒前
Owen应助小先生采纳,获得10
4秒前
浪荡胭脂马完成签到,获得积分10
5秒前
5秒前
大晨发布了新的文献求助10
5秒前
GOD伟完成签到,获得积分10
5秒前
SXX发布了新的文献求助10
5秒前
暮潇牧笑完成签到,获得积分10
6秒前
黄小佳发布了新的文献求助10
6秒前
kkkkkkk_发布了新的文献求助20
7秒前
哎呦喂完成签到 ,获得积分10
7秒前
搜集达人应助笨笨的傲芙采纳,获得30
8秒前
Jasper应助耳机单蹦采纳,获得10
8秒前
krkr发布了新的文献求助10
8秒前
9秒前
周周完成签到 ,获得积分10
9秒前
菜菜驳回了wanci应助
9秒前
小蘑菇应助贝肯尼采纳,获得10
9秒前
小董不懂发布了新的文献求助10
9秒前
10秒前
黄阿鹏发布了新的文献求助10
10秒前
10秒前
在水一方应助DRShan采纳,获得10
11秒前
不问归期的风完成签到,获得积分0
11秒前
12秒前
高分求助中
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Дружба 友好报 (1957-1958) 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 600
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 600
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3106352
求助须知:如何正确求助?哪些是违规求助? 2757369
关于积分的说明 7644699
捐赠科研通 2411634
什么是DOI,文献DOI怎么找? 1279512
科研通“疑难数据库(出版商)”最低求助积分说明 617805
版权声明 599271