Regulation of maltase synthesis in Saccharomyces carlsbergensis

The induction of maltase (EC 3.2.1.20) by its inducer maltose in a strain of the yeast Saccharomyces carlsbergensis carrying a functional MAL locus is regulated at the level of transcription. Preceding the synthesis of increased levels of maltase is the de novo synthesis of maltase-specific RNA sequences. This was detected by determining the level of maltase mRNA by DNA-RNA hybridizations by using a maltase structural gene DNA sequence probe and by assaying functional maltase mRNA by in vitro RNA-directed synthesis of immunologically reactive maltase. Once maltase has accumulated, late in induction, further synthesis of the enzyme is inhibited, as reflected by reduced levels of the mRNA that encodes maltase.