胚泡
外胚层
生物
胚胎干细胞
细胞生物学
诱导多能干细胞
胚胎发生
内细胞团
胚胎
转录组
遗传学
基因
原肠化
基因表达
作者
Yong Fan,Zheying Min,Samhan Alsolami,Zhenglai Ma,E. Zhang,Wei Chen,Ke Zhong,Wendi Pei,Xiangjin Kang,Pu-Yao Zhang,Yongliang Wang,Yingying Zhang,Linfeng Zhan,Haiying Zhu,Chenrui An,Rong Li,Jie Qiao,Tao Tan,Mo Li,Yang Yu
标识
DOI:10.1038/s41421-021-00316-8
摘要
Abstract Human blastocysts are comprised of the first three cell lineages of the embryo: trophectoderm, epiblast and primitive endoderm, all of which are essential for early development and organ formation. However, due to ethical concerns and restricted access to human blastocysts, a comprehensive understanding of early human embryogenesis is still lacking. To bridge this knowledge gap, a reliable model system that recapitulates early stages of human embryogenesis is needed. Here we developed a three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts. Furthermore, EPS-blastoids are similar with human embryos that were cultured for 8 or 10 days in vitro, in terms of embryonic structures, cell lineages and transcriptomic profiles. In conclusion, we developed a scalable system to mimic human blastocyst development, which can potentially facilitate the study of early implantation failure that induced by developmental defects at early stage.
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