九氟化硫
鲎
重组DNA
细菌
生物
细胞培养
化学
微生物学
分子生物学
夜蛾
生物化学
基因
遗传学
古生物学
标识
DOI:10.1023/a:1026764514737
摘要
Limulus Factor C is a potent antagonist of endotoxin from Gram-negative bacteria. A fusion construct containing full length Factor C has been cloned intoSpodoptera frugiperda Sf9 cell. Stable Sf9 cell transfectants were obtained using Zeocin selection for 2 weeks. The recombinant Factor C (rFC) was secreted into the culture medium at 9 mg l−1. Both the crude and partially purified rFC were able to detect lipid A at 10 pg ml−1 in an ELISA-based lipid A binding assay.
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