Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

拟南芥 生物 基因 遗传学 雄蕊 微阵列分析技术 突变体 基因表达谱 基因表达 小孢子 拟南芥 基因表达调控 植物 花粉
作者
Márcio Alves‐Ferreira,Frank Wellmer,Aline Banhara,Vijaya B. Kumar,José Luis Riechmann,Elliot M. Meyerowitz
出处
期刊:Plant Physiology [Oxford University Press]
卷期号:145 (3): 747-762 被引量:119
标识
DOI:10.1104/pp.107.104422
摘要

Abstract To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.
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