Urine Derived Cells are a Potential Source for Urological Tissue Reconstruction

No AccessJournal of UrologyInvestigative Urology1 Nov 2008Urine Derived Cells are a Potential Source for Urological Tissue Reconstructionis companion ofIndications for Preoperative Prostate Biopsy in Patients Undergoing Radical Cystoprostatectomy for Bladder CancerUrethrectomy Following Cystectomy for Bladder Cancer in Men: Practice Patterns and Impact on SurvivalSerosa Lined and Tapered Ileum as Primary and Secondary Continence Mechanism for Various Catheterizable Pouches Yuanyuan Zhang, Elena McNeill, Hong Tian, Shay Soker, Karl-Erik Andersson, James J. Yoo, and Anthony Atala Yuanyuan ZhangYuanyuan Zhang More articles by this author , Elena McNeillElena McNeill More articles by this author , Hong TianHong Tian More articles by this author , Shay SokerShay Soker More articles by this author , Karl-Erik AnderssonKarl-Erik Andersson More articles by this author , James J. YooJames J. Yoo More articles by this author , and Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2008.07.023AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Contemporary approaches to tissue engineering and cell therapy for urinary tract reconstruction require invasive tissue biopsies to obtain autologous cells. However, these procedures are associated with potential complications. We determined whether the cells present in urine have characteristics of normal bladder cells and investigated their potential uses for urological reconstructive procedures. Materials and Methods: A total of 55 urine samples were collected from 15 healthy individuals and 8 patients with vesicoureteral reflux. Urine derived cells were isolated, expanded and tested for progenitor and differentiated cell specific markers using flow cytometry, immunofluorescence and Western immunoblotting. The chromosomal stability of cultured urine derived cells was determined by karyotype analysis. Results: Clones were successfully established from primary cultures of urine derived cells. Isolated cells showed 3 phenotypes, including fully differentiated, differentiating and progenitor-like cells. Some urine derived cells stained positive for the surface markers c-Kit, SSEA4, CD105, CD73, CD91, CD133 and CD44. Two to 7 cells per 100 ml urine were multipoint progenitors that could expand extensively in culture. Single progenitor cells had the ability to differentiate into the cell lineages expressing urothelial, smooth muscle, endothelial and interstitial cell markers. The expression of lineage markers was characterized by Western blot and immunofluorescence analysis. Urine derived cells also maintained a normal karyotype after serial culture. Conclusions: A subpopulation of cells isolated from urine had progenitor cell features and the potential to differentiate into several bladder cell lineages. Urine derived cells could serve as an alternative cell source for urinary tract tissue engineering and reconstruction. References 1 : Tissue- engineered autologous bladders for patients needing cystoplasty. Lancet2006; 367: 1241. Google Scholar 2 : De novo reconstitution of a functional mammalian urinary bladder by tissue engineering. Nat Biotechnol1999; 17: 149. Google Scholar 3 : Bladder reconstitution with bone marrow derived stem cells seeded on small intestinal submucosa improves morphological and molecular composition. J Urol2005; 174: 353. Link, Google Scholar 4 : Growth of bone marrow stromal cells on small intestinal submucosa: an alternative cell source for tissue engineered bladder. BJU Int2005; 96: 1120. 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Google Scholar Institute for Regenerative Medicine, School of Medicine, Wake Forest University, Winston-Salem, North Carolina© 2008 by American Urological AssociationFiguresReferencesRelatedDetailsCited byWu S, Wang Z, Bharadwaj S, Hodges S, Atala A and Zhang Y (2011) Implantation of Autologous Urine Derived Stem Cells Expressing Vascular Endothelial Growth Factor for Potential Use in Genitourinary ReconstructionJournal of Urology, VOL. 186, NO. 2, (640-647), Online publication date: 1-Aug-2011.Related articlesJournal of Urology17 Sep 2008Indications for Preoperative Prostate Biopsy in Patients Undergoing Radical Cystoprostatectomy for Bladder CancerJournal of Urology17 Sep 2008Urethrectomy Following Cystectomy for Bladder Cancer in Men: Practice Patterns and Impact on SurvivalJournal of Urology18 Sep 2008Serosa Lined and Tapered Ileum as Primary and Secondary Continence Mechanism for Various Catheterizable Pouches Volume 180Issue 5November 2008Page: 2226-2233 Advertisement Copyright & Permissions© 2008 by American Urological AssociationKeywordsbladdertissue engineeringstem cellsurineurotheliumAcknowledgmentsDr. Peilin Li assisted with chromosome and karyotype analysis, Dr. Yujiang provided urine cell collection and culture, Jennifer Olson assisted with the manuscript and Dr. Mark Furth provided comments. Mouse anti-human CK 13, uroplakin Ia, calponin, desmin and peroxidase labeled goat anti-mouse IgG were obtained from Santa Cruz Biotechnology, Santa Cruz, California.MetricsAuthor Information Yuanyuan Zhang More articles by this author Elena McNeill More articles by this author Hong Tian More articles by this author Shay Soker More articles by this author Karl-Erik Andersson More articles by this author James J. Yoo More articles by this author Anthony Atala More articles by this author Expand All Advertisement PDF downloadLoading ...