一氧化氮
恶臭假单胞菌
一氧化氮合酶
生物膜
细菌
枯草芽孢杆菌
生物
微生物学
生物化学
异源的
内生
化学
基因
遗传学
内分泌学
作者
Pulin Liu,Qiaoyun Huang,Wenli Chen
出处
期刊:Canadian Journal of Microbiology
[Canadian Science Publishing]
日期:2012-03-01
卷期号:58 (3): 336-344
被引量:13
摘要
The nitric oxide synthase gene from Bacillus subtilis was heterologously expressed in Pseudomonas putida using a broad-host expression vector. Both the performance of the nitric oxide-specific fluorescent probe and the quantitative assessment of the nitric oxide end products demonstrated the generation of nitric oxide. The endogenous nitric oxide increased the motility of P. putida and decreased the capacity of P. putida and other multispecies bacteria to develop biofilms. On the practical side, endogenous nitric oxide offers an advantage in generating continuous, controllable levels of nitric oxide, which suggests a new method to use nitric oxide in the control of biofilms.
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