器官发生
丝氨酸
磷酸化
信号转导
莲藕
生物
根瘤
激酶
蛋白激酶结构域
细胞生物学
生物化学
遗传学
共生
基因
突变体
细菌
作者
Nikolaj B. Abel,Malita Malou Malekzadeh Nørgaard,Simon B. Hansen,Kira Gysel,Ignacio Arribas Díez,Ole N. Jensen,Jens Stougaard,Kasper R. Andersen
标识
DOI:10.1073/pnas.2311522121
摘要
Symbiosis receptor-like kinase SYMRK is required for root nodule symbiosis between legume plants and nitrogen-fixing bacteria. To understand symbiotic signaling from SYMRK, we determined the crystal structure to 1.95 Å and mapped the phosphorylation sites onto the intracellular domain. We identified four serine residues in a conserved "alpha-I" motif, located on the border between the kinase core domain and the flexible C-terminal tail, that, when phosphorylated, drives organogenesis. Substituting the four serines with alanines abolished symbiotic signaling, while substituting them with phosphorylation-mimicking aspartates induced the formation of spontaneous nodules in the absence of bacteria. These findings show that the signaling pathway controlling root nodule organogenesis is mediated by SYMRK phosphorylation, which may help when engineering this trait into non-legume plants.
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