Lactobacillus paracasei JY062 alleviates glucolipid metabolism disorders by adipoinsular axis and gut microbiota

Abstract High-glucose-fat diet-induced glucolipid metabolism disorder has become a global epidemic disease. Probiotic intervention is a new strategy to alleviate metabolic syndrome. The protective effect and mechanism of L. paracasei JY062 on high-glucose-fat diets mice were investigated. After 7 weeks L. paracasei JY062 intervention, the body weight of mice in the LPH group (28.07 ± 1.07 g) was significantly lower than that of HFD group (32.03 ± 0.89 g) ( P < 0.05). Among three doses of JY062, 10 8 and 10 9 CFU/mL JY062 showed the strong ability to relieve blood glucose disorders, blood lipid disorders, tissue damage. LPH group had the best effect, significantly ( P < 0.05) decreased the TC (40.11%), TG (42.46%), LDL-C (39.38%), leptin (54.34%), insulin (39.95%), FFA (40.68%) concentrations and increased ( P < 0.05) the HDL-C (1.05 times), adiponectin (1.53 times) and GLP-1 (1.35 times) level compared to HFD group mice. JY062 could activate the APN-AMPK pathway, increase AdipoQ, AMPK GLUT-4 and PGC-1αmRNA expression, and decrease SREBP-1c, ACC and FAS mRNA expression. L. paracasei JY062 intervention decreased the relative abundance of harmful bacteria and increased the relative abundance of beneficial bacteria, restored the imbalance of gut microbiota homeostasis caused by high-glucose-fat diet. L. paracasei JY062 could also increase the content of SCFAs in the intestines, especially butyric acid.