少动鞘氨醇单胞菌
结冷胶
食品科学
玉米浆
化学
生产成本
制浆造纸工业
鞘脂单胞菌属
生物技术
发酵
细菌
生物化学
生物
工程类
基因
机械工程
遗传学
16S核糖体RNA
作者
Jin Huang,Shengquan Zhu,Chuanbao Li,Chenglin Zhang,Yizhi Ji
标识
DOI:10.1080/10826068.2019.1692215
摘要
Gellan gum, produced by Sphingomonas paucimobilis, is increasingly used in food and pharmaceutical industries as stabilizing, emulsifying, texturing and gelling agents. However, its high production costs may limit its full commercial potential. Therefore, in this study, we investigated ways to reduce gellan gum production costs and improve yields. We first revealed corn steep liquor (CSL) as a cost-effective nutrient source that can improve gellan gum yields. We then systematically optimized culture conditions even further, and revealed that the addition of Triton X-100 surfactant and selected inorganic nitrogen sources improved gellan gum production. Under our optimized conditions (glucose 33.75 g/L, CSL 10 g/L, urea 2.5 g/L, MgSO4 1.08 g/L, KH2PO4 3.24 g/L, K2SO4 1 g/L and Triton X-100 0.75 g/L), we yielded a maximum concentration of 14.41 g/L, which was about 1.5-fold higher than non-optimized CSL-based medium. Our findings highlight the use of CSL as a cost effective and promising nutrient source for industrial production of gellan gum.
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