细胞培养中氨基酸的稳定同位素标记
PI3K/AKT/mTOR通路
细胞周期
细胞凋亡
细胞周期检查点
定量蛋白质组学
细胞生物学
生物
RPTOR公司
蛋白质组学
程序性细胞死亡
核糖体生物发生
细胞生长
化学
信号转导
生物化学
核糖体
核糖核酸
基因
作者
Xia Ji,Qiang Luo,Shengbin Huang,Fuquan Jiang,Lin Wang,Guanghui Wang,Jingjing Xie,Jie Liu,Xu Yang
出处
期刊:Chinese Journal of Cancer Research
[Chinese Journal of Cancer Research]
日期:2019-01-01
卷期号:31 (2): 375-388
被引量:6
标识
DOI:10.21147/j.issn.1000-9604.2019.02.12
摘要
The present study aimed to investigate the molecular events in alisol B 23-acetate (ABA) cytotoxic activity against a liver cancer cell line.First, we employed a quantitative proteomics approach based on stable isotope labeling by amino acids in cell culture (SILAC) to identify the different proteins expressed in HepG2 liver cancer cells upon exposure to ABA. Next, bioinformatics analyses through DAVID and STRING on-line tools were used to predict the pathways involved. Finally, we applied functional validation including cell cycle analysis and Western blotting for apoptosis and mTOR pathway-related proteins to confirm the bioinformatics predictions.We identified 330 different proteins with the SILAC-based quantitative proteomics approach. The bioinformatics analysis and the functional validation revealed that the mTOR pathway, ribosome biogenesis, cell cycle, and apoptosis pathways were differentially regulated by ABA. G1 cell cycle arrest, apoptosis and mTOR inhibition were confirmed.ABA, a potential mTOR inhibitor, induces the disruption of ribosomal biogenesis. It also affects the mTOR-MRP axis to cause G1 cell cycle arrest and finally leads to cancer cell apoptosis.
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