放线杆菌
中间普氏菌
牙龈卟啉单胞菌
牙周炎
微生物学
实时聚合酶链反应
连续稀释
慢性牙周炎
生物
牙密螺旋体
细菌
普雷沃菌属
16S核糖体RNA
医学
病理
牙科
遗传学
基因
替代医学
作者
Claudia Nonnenmacher,Alexander H. Dalpke,Reinier Mutters,Klaus Heeg
标识
DOI:10.1016/j.mimet.2004.06.006
摘要
Specific bacteria are believed to play an important role in chronic periodontitis, yet the significance of their relative numbers in initiation and progress of the disease is still unclear. We report here the development of a sensitive, quantitative PCR technique for enumerating Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Dialister pneumosintes (Dp) and Micromonas micros (Mm) as well as total eubacteria in subgingival plaque samples from subjects with periodontitis. Quantification was performed with specific 16S rRNA target sequences with double fluorescence labeled probes and serial dilutions of plasmid standard by real-time PCR. This method showed a broad quantification range from 102 to 108 and accurate sensitivity and specificity. Fifty subgingival plaque samples from periodontitis patients and 33 from periodontally healthy subjects were subsequently examined. Higher levels of total bacteria numbers, Aa, Pg, Dp and Mm were found in samples from periodontitis subjects in comparison to samples from periodontally healthy subjects. Quantitative real-time PCR thus provides a reliable and valuable method for quantification of periodontopathogens in subgingival plaque samples.
科研通智能强力驱动
Strongly Powered by AbleSci AI